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CALCOCO2 Antibody

OAAN02243
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCALCOCO2
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    CALCOCO2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    WB: 1:500 - 1:2,000 IHC: 1:50 - 1:200
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Format
    Liquid. PBS with 0.02% NaN3, 50% Glycerol, pH 7.3
  • Gene ID10241
  • Target name
    CALCOCO2
  • Target description
    calcium binding and coiled-coil domain 2
  • Target synonyms
    NDP52, calcium-binding and coiled-coil domain-containing protein 2, antigen nuclear dot 52 kDa protein, nuclear domain 10 protein 52, nuclear domain 10 protein NDP52, nuclear dot protein 52
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13137
  • Protein Name
    Calcium-binding and coiled-coil domain-containing protein 2
  • Scientific Description
    This gene encodes a coiled-coil domain-containing protein. The encoded protein functions as a receptor for ubiquitin-coated bacteria and plays an important role in innate immunity by mediating macroautophagy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDP52/CALCOCO2 using anti-NDP52/CALCOCO2 antibody (A05876-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human U20S wholel cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDP52/CALCOCO2 antigen affinity purified polyclonal antibody (Catalog # A05876-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDP52/CALCOCO2 at approximately 52KD. The expected band size for NDP52/CALCOCO2 is at 52KD.
Product group Antibodies
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Anti-NDP52/CALCOCO2 Antibody Picoband(r)A05876-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCALCOCO2
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TargetCALCOCO2
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Western blot All lanes: CALCOCO2 antibody at 3.36ug/ml Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 53, 48, 55, 56, 44 kDa Observed band size: 56 kDa
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Immunohistochemistry analysis in human testis and pancreas tissues using HPA023195 antibody. Corresponding CALCOCO2 RNA-seq data are presented for the same tissues.
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Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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