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ProductsBack/CALCOCO2 Mouse Monoclonal Antibody [Clone ID: OTI7A7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2 (1:2000).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2 (1:2000).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2 (1:2000).

CALCOCO2 Mouse Monoclonal Antibody [Clone ID: OTI7A7]

CF502106
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman
TargetCALCOCO2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CALCOCO2 Mouse Monoclonal Antibody [Clone ID: OTI7A7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI7A7
  • Gene ID10241
  • Target name
    CALCOCO2
  • Target description
    calcium binding and coiled-coil domain 2
  • Target synonyms
    NDP52, calcium-binding and coiled-coil domain-containing protein 2, antigen nuclear dot 52 kDa protein, nuclear domain 10 protein 52, nuclear domain 10 protein NDP52, nuclear dot protein 52
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ13137
  • Protein Name
    Calcium-binding and coiled-coil domain-containing protein 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) CALCOCO2 mouse monoclonal antibody, clone OTI7A7 (formerly 7A7)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of NDP52/CALCOCO2 using anti-NDP52/CALCOCO2 antibody (A05876-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human U20S wholel cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDP52/CALCOCO2 antigen affinity purified polyclonal antibody (Catalog # A05876-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDP52/CALCOCO2 at approximately 52KD. The expected band size for NDP52/CALCOCO2 is at 52KD.
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