More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Calpain 2 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain 2 Polyclonal Antibody, Unconjugated (bs-1599R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain 2 Polyclonal Antibody, Unconjugated (bs-1599R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Calpain 2 Polyclonal Antibody, Unconjugated (bs-1599R) at 1:400 overnight at 4°C, DAB staining.

Calpain 2 Polyclonal Antibody

BS-1599R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCAPN2
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    Calpain 2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID824
  • Target name
    CAPN2
  • Target description
    calpain 2
  • Target synonyms
    CANP2, CANPL2, CANPml, mCANP, calpain-2 catalytic subunit, CANP 2, M-calpain, calcium-activated neutral proteinase 2, calpain 2, (m/II) large subunit, calpain 2, large [catalytic] subunit, calpain 2, large subunit, calpain M-type, calpain, large polypeptide L2, millimolar-calpain
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Biomatik
Capn2 Polyclonal AntibodyCAC08282
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
TargetCAPN2
  • SizePrice
Product group Antibodies
Assay Genie
[KO Validated] Calpain 2 AntibodyCAB1861
TargetCAPN2
  • SizePrice
Figure 1. Western blot analysis of Calpain 2 using anti-Calpain 2 antibody (A03492). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpain 2 antigen affinity purified polyclonal antibody (Catalog # A03492) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpain 2 at approximately 80 kDa. The expected band size for Calpain 2 is at 80 kDa.
Product group Antibodies
Boster Bio
Anti-Calpain 2/CAPN2 Antibody Picoband(r)A03492-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetCAPN2
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-Calpain 2 AntibodyA283451
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CAPN2 Antibody144-01861
ApplicationsImmunoFluorescence, Western Blot
TargetCAPN2
  • SizePrice
Product group Antibodies
References
GeneTex
Calpain 2 antibody [N2C1-2], InternalGTX111809
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetCAPN2
  • SizePrice
Product group Antibodies
MedChem Express
Calpain 2 Antibody (YA572, Rabbit)HY-P80444
TargetCAPN2
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CAPN2 / Calpain 2 / M-Calpain AntibodyLS-C667728
ApplicationsWestern Blot
TargetCAPN2
  • SizePrice
Immunohistochemistry analysis in human lung and liver tissues using HPA024470 antibody. Corresponding CAPN2 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-CAPN2 AntibodyHPA024470
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCAPN2
  • SizePrice
Product group Antibodies
Nordic-MUbio
Mouse anti Human Calpain I, II (small subunit), conjugated with agaroseX1011
ApplicationsImmunoPrecipitation, Western Blot
TargetCAPN2
  • SizePrice