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Western Blot Positive WB detected in: 293T whole cell lysate All lanes: CAPN2 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80, 72 kDa Observed band size: 80 kDa
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: CAPN2 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80, 72 kDa Observed band size: 80 kDa
Western Blot Positive WB detected in: 293T whole cell lysate All lanes: CAPN2 antibody at 3.4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80, 72 kDa Observed band size: 80 kDa

CAPN2 Antibody

CSB-PA004496LA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCAPN2
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Overview

  • Supplier
    Cusabio
  • Product Name
    CAPN2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID824
  • Target name
    CAPN2
  • Target description
    calpain 2
  • Target synonyms
    calcium-activated neutral proteinase 2; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; calpain M-type; calpain, large polypeptide L2; calpain-2 catalytic subunit; CANP 2; CANP2; CANPL2; CANPml; M-calpain; mCANP; millimolar-calpain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17655
  • Protein Name
    Calpain-2 catalytic subunit
  • Scientific Description
    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calpain 2 using anti-Calpain 2 antibody (A03492). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat kidney tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse kidney tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calpain 2 antigen affinity purified polyclonal antibody (Catalog # A03492) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calpain 2 at approximately 80 kDa. The expected band size for Calpain 2 is at 80 kDa.
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