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WB analysis of HepG2 lysates using GTX88377 Calponin 2 antibody, Internal. Dilution : 0.03microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of HepG2 lysates using GTX88377 Calponin 2 antibody, Internal. Dilution : 0.03microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of HepG2 lysates using GTX88377 Calponin 2 antibody, Internal. Dilution : 0.03microg/ml Loading : 35microg protein in RIPA buffer

Calponin 2 antibody, Internal

GTX88377
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCNN2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Calponin 2 antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.03-0.1microg/ml. IHC-P: 2.5microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1265
  • Target name
    CNN2
  • Target description
    calponin 2
  • Target synonyms
    calponin H2, smooth muscle; calponin-2; neutral calponin
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDQ99439
  • Protein Name
    Calponin-2
  • Scientific Description
    The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Several pseudogenes of this gene have been identified, and are present on chromosomes 1, 2, 3, 6, 9, 11, 13, 15, 16, 21 and 22. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calponin 2/CNN2 using anti-Calponin 2/CNN2 antibody (A08680-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calponin 2/CNN2 antigen affinity purified polyclonal antibody (Catalog # A08680-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calponin 2/CNN2 at approximately 34 kDa. The expected band size for Calponin 2/CNN2 is at 34 kDa.
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