Camptothecin

Biorbyt

Camptothecin

16

1. Dissolve staurosporine powder in tissue culture grade DMSO to obtain a 1 mM staurosporine concentration., 2. Prepare 50 - 100 microL aliquots of the DMSO solubilized staurosporine stock solution and store them frozen at < -20°C. A frozen vial of staurosporine may only be rethawed 2X before it must be discarded., 3. Spike cell cultures at a staurosporine concentration of 1 microM in the cell culture media. This equates to a 1 microL spike of the 1 mM staurosporine stock per mL of the cell culture suspension. This concentration works well for inducing many cell lines including HL-60 and Jurkat cells when using a 4-5 hour 37°C incubation period. Typical cell suspension concentrations that have been used for this staurosporine induction protocol range from 1 x 105 - 1 x 106 cells/mL., 4. Perform time course studies on your particular cell line to ascertain the optimal staurosporine concentration and exposure time required to achieve good apoptosis induction levels in your experimental system., 5. Proceed with your experimental apoptosis induction model system.

Research Use Only

Camptothecin, a cytotoxic plant alkaloid with antitumor properties, is a prototypic DNA topoisomerase I inhibitor. Use camptothecin to create positive controls in apoptosis detection experiments.

-20°C

12352200