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ProductsBack/Carboxylesterase 1 / CES1 Antibody (aa33-82, Biotin)

Carboxylesterase 1 / CES1 Antibody (aa33-82, Biotin)

LS-C442891
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Guinea Pig, Human, Monkey, Primate, Rat
TargetCES1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Carboxylesterase 1 / CES1 Antibody (aa33-82, Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot
  • Applications Supplier
    WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID1066
  • Target name
    CES1
  • Target description
    carboxylesterase 1
  • Target synonyms
    ACAT, CE-1, CEH, CES2, HMSE, HMSE1, PCE-1, REH, SES1, TGH, hCE-1, liver carboxylesterase 1, acyl coenzyme A:cholesterol acyltransferase, brain carboxylesterase hBr1, carboxylesterase 1 (monocyte/macrophage serine esterase 1), carboxylesterase 2 (liver), cholesteryl ester hydrolase, cocaine carboxylesterase, egasyn, human monocyte/macrophage serine esterase 1, methylumbelliferyl-acetate deacetylase 1, monocyte/macrophage serine esterase, retinyl ester hydrolase, serine esterase 1, triacylglycerol hydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Canine, Guinea Pig, Human, Monkey, Primate, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CES1 using anti-CES1 antibody (A01741-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CES1 antigen affinity purified polyclonal antibody (Catalog # A01741-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CES1 at approximately 62 kDa. The expected band size for CES1 is at 62 kDa.
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