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ProductsBack/Carboxypeptidase A (CPA1) Mouse Monoclonal Antibody [Clone ID: OTI4F1]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CPA1 (RC202720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CPA1. Positive lysates LY419696 (100ug) and LC419696 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CPA1 (RC202720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CPA1. Positive lysates LY419696 (100ug) and LC419696 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CPA1 (RC202720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CPA1. Positive lysates LY419696 (100ug) and LC419696 (20ug) can be purchased separately from OriGene.

Carboxypeptidase A (CPA1) Mouse Monoclonal Antibody [Clone ID: OTI4F1]

CF500170
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCPA1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Carboxypeptidase A (CPA1) Mouse Monoclonal Antibody [Clone ID: OTI4F1]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4F1
  • Gene ID1357
  • Target name
    CPA1
  • Target description
    carboxypeptidase A1
  • Target synonyms
    CPA, carboxypeptidase A1, carboxypeptidase A1 (pancreatic), pancreatic carboxypeptidase A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15085
  • Protein Name
    Carboxypeptidase A1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) CPA1 mouse monoclonal antibody, clone OTI4F1 (formerly 4F1)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of Carboxypeptidase A/CPA1 using anti-Carboxypeptidase A/CPA1 antibody (A05985-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Carboxypeptidase A/CPA1 antigen affinity purified polyclonal antibody (Catalog # A05985-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Carboxypeptidase A/CPA1 at approximately 47 kDa. The expected band size for Carboxypeptidase A/CPA1 is at 47 kDa.
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