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ProductsBack/Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb

Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb

A9308
ABclonal
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCSNK1A1
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Overview

  • Supplier
    ABclonal
  • Product Name
    Casein Kinase 1 alpha (CSNK1A1) Rabbit mAb
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID1452
  • Target name
    CSNK1A1
  • Target description
    casein kinase 1 alpha 1
  • Target synonyms
    CK1, CK1a, CKIa, HEL-S-77p, HLCDGP1, PRO2975, casein kinase I isoform alpha, CKI-alpha, clock regulator kinase, down-regulated in lung cancer, epididymis secretory sperm binding protein Li 77p
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP48729
  • Protein Name
    Casein kinase I isoform alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CSNK1A1 using anti-CSNK1A1 antibody (PB9693). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Mouse Kidney Tissue Lysate, Lane 4: HELA Whole Cell Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK1A1 antigen affinity purified polyclonal antibody (Catalog # PB9693) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK1A1 at approximately 39KD. The expected band size for CSNK1A1 is at 39KD.
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