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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA004539LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA004539LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA004539LA01HU at dilution of 1:100

CASK Antibody

CSB-PA004539LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCASK
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Overview

  • Supplier
    Cusabio
  • Product Name
    CASK Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8573
  • Target name
    CASK
  • Target description
    calcium/calmodulin dependent serine protein kinase
  • Target synonyms
    CAGH39; calcium/calmodulin-dependent serin protein kinase; calcium/calmodulin-dependent serine protein kinase (MAGUK family); calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase; CAMGUK; CMG; FGS4; hCASK; LIN2; MICPCH; MRXSNA; peripheral plasma membrane protein CASK; protein lin-2 homolog; TNRC8; trinucleotide repeat containing 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14936
  • Protein Name
    Peripheral plasma membrane protein CASK
  • Scientific Description
    Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CASK using anti-CASK antibody (A02468-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASK antigen affinity purified polyclonal antibody (Catalog # A02468-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASK at approximately 120 kDa. The expected band size for CASK is at 120 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CASK/CAMGUK Polyclonal Antibody, Unconjugated (bs-11338R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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