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CASK antibody

GTX111513
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCASK
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Overview

  • Supplier
    GeneTex
  • Product Name
    CASK antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8573
  • Target name
    CASK
  • Target description
    calcium/calmodulin dependent serine protein kinase
  • Target synonyms
    CAGH39, CAMGUK, CMG, FGS4, LIN2, MICPCH, MRXSNA, TNRC8, hCASK, peripheral plasma membrane protein CASK, calcium/calmodulin-dependent serin protein kinase, calcium/calmodulin-dependent serine protein kinase (MAGUK family), calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase, protein lin-2 homolog, trinucleotide repeat containing 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14936
  • Protein Name
    Peripheral plasma membrane protein CASK
  • Scientific Description
    The CASK gene encodes a calcium/calmodulin-dependent serine protein kinase that is a member of the membrane-associated guanylate kinase (MAGUK) protein family. MAGUKs are scaffolding proteins associated with intercellular junctions (Atasoy et al., 2007 [PubMed 17287346]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CASK using anti-CASK antibody (A02468-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASK antigen affinity purified polyclonal antibody (Catalog # A02468-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASK at approximately 120 kDa. The expected band size for CASK is at 120 kDa.
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