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ProductsBack/caspase-1 p20 (D-4) Alexa Fluor(r) 546

caspase-1 p20 (D-4) Alexa Fluor(r) 546

SC-398715AF546
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityMouse, Rat
TargetCasp1
Price on request
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    caspase-1 p20 (D-4) Alexa Fluor(r) 546
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 546
  • Gene ID12362
  • Target name
    Casp1
  • Target description
    caspase 1
  • Target synonyms
    ICE, Il1bc, caspase-1, CASP-1, IL-1 beta-converting enzyme, IL-1B converting enzyme, IL-1BC, interleukin-1 beta convertase, interleukin-1 beta-converting enzyme, p45
  • Host
    Mouse
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

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WB analysis of various samples using GTX11701 Caspase 1 p20 subunit antibody. Lane 1 : rat brain tissue lysate Lane 2 : rat spleen tissue lysate Lane 3 : mouse brain tissue lysate Lane 4 : mouse spleen tissue lysate Lane 5 : mouse testis tissue lysate
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Figure 1. Western blot analysis of CASP1 using anti-CASP1 antibody (PA1440-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP1 antigen affinity purified polyclonal antibody (Catalog # PA1440-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CASP1 at approximately 46 kDa. The expected band size for CASP1 is at 46 kDa.
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