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Caspase-3 Monoclonal Antibody

CAB17900
Assay Genie
ReactivityHuman
Product group Antibodies
TargetHGS
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Caspase-3 Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID9146
  • Target name
    HGS
  • Target description
    hepatocyte growth factor-regulated tyrosine kinase substrate
  • Target synonyms
    HRS, hepatocyte growth factor-regulated tyrosine kinase substrate, human growth factor-regulated tyrosine kinase substrate, protein pp110
  • Host
    Rabbit
  • Protein IDO14964
  • Protein Name
    Hepatocyte growth factor-regulated tyrosine kinase substrate
  • Shelf life instruction
    12 months
  • Source
    Mouse
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HGS using anti-HGS antibody (A01174-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HGS antigen affinity purified polyclonal antibody (Catalog # A01174-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HGS at approximately 110-115KD. The expected band size for HGS is at 110-115KD.
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