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ProductsBack/Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Polyclonal Antibody

Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Polyclonal Antibody

CAU34448
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetHGS
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Overview

  • Supplier
    Biomatik
  • Product Name
    Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9146
  • Target name
    HGS
  • Target description
    hepatocyte growth factor-regulated tyrosine kinase substrate
  • Target synonyms
    HRS, hepatocyte growth factor-regulated tyrosine kinase substrate, human growth factor-regulated tyrosine kinase substrate, protein pp110
  • Host
    Rabbit
  • Protein IDO14964
  • Protein Name
    Hepatocyte growth factor-regulated tyrosine kinase substrate
  • Scientific Description
    The Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate (HGS) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HGS using anti-HGS antibody (A01174-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HGS antigen affinity purified polyclonal antibody (Catalog # A01174-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HGS at approximately 110-115KD. The expected band size for HGS is at 110-115KD.
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