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Cathepsin K (Marker of Tumor Invasiveness)(CTSK/2791), CF647 conjugate, 0.1mg/mL [26628-22-8]

Cathepsin K (Marker of Tumor Invasiveness)(CTSK/2791), CF647 conjugate, 0.1mg/mL [26628-22-8]

BNC472791
Biotium
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCTSK
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Overview

  • Supplier
    Biotium
  • Product Name
    Cathepsin K (Marker of Tumor Invasiveness)(CTSK/2791), CF647 conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CTSK/2791
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID1513
  • Target name
    CTSK
  • Target description
    cathepsin K
  • Target synonyms
    cathepsin K; cathepsin O; cathepsin O1; cathepsin O2; cathepsin X; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP43235
  • Protein Name
    Cathepsin K
  • Scientific Description
    Cathepsin K is a lysosomal and secreted cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cathepsin K using anti-Cathepsin K antibody (PB9856). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin K antigen affinity purified polyclonal antibody (Catalog # PB9856) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin K at approximately 45 kDa. The expected band size for Cathepsin K is at 40 kDa.
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