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Western blot All lanes: CTSK antibody at 8ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Rat brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa
Western blot All lanes: CTSK antibody at 8ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Rat brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa
Western blot All lanes: CTSK antibody at 8ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Rat brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37 kDa Observed band size: 37 kDa

CTSK Antibody

CSB-PA006192ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetCTSK
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Overview

  • Supplier
    Cusabio
  • Product Name
    CTSK Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1513
  • Target name
    CTSK
  • Target description
    cathepsin K
  • Target synonyms
    cathepsin K; cathepsin O; cathepsin O1; cathepsin O2; cathepsin X; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43235
  • Protein Name
    Cathepsin K
  • Scientific Description
    Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cathepsin K using anti-Cathepsin K antibody (PB9856). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin K antigen affinity purified polyclonal antibody (Catalog # PB9856) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin K at approximately 45 kDa. The expected band size for Cathepsin K is at 40 kDa.
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