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Cathepsin K Polyclonal Antibody

BS-1611R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCTSK
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Cathepsin K Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(WB=1:500-2000), IHC-P(IHC-P=1:100-500), IHC-F(IHC-F=1:100-500), IF(IF=1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1513
  • Target name
    CTSK
  • Target description
    cathepsin K
  • Target synonyms
    cathepsin K; cathepsin O; cathepsin O1; cathepsin O2; cathepsin X; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Ma Q et al., 2019, Am J Transl Res
    Read more
  • A novel regulatory role of TRAPPC9 in L-plastin-mediated osteoclast actin ring formation. Hussein NJ et al., 2020 Jan, J Cell Biochem
    Read more
  • Decreased cathepsin K levels in human atherosclerotic plaques are associated with plaque instability. Zhao H et al., 2017 Oct, Exp Ther Med
    Read more

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Figure 1. Western blot analysis of Cathepsin K using anti-Cathepsin K antibody (PB9856). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: 22RV1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin K antigen affinity purified polyclonal antibody (Catalog # PB9856) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin K at approximately 45 kDa. The expected band size for Cathepsin K is at 40 kDa.
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