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Western Blot analysis of Mouse liver and Human fetal lung tissue, hela cell and Mouse kidney tissue, Human brain malignant glioma tissue and K562 cell using CBR1 Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of Mouse liver and Human fetal lung tissue, hela cell and Mouse kidney tissue, Human brain malignant glioma tissue and K562 cell using CBR1 Polyclonal Antibody at dilution of 1:1000
Western Blot analysis of Mouse liver and Human fetal lung tissue, hela cell and Mouse kidney tissue, Human brain malignant glioma tissue and K562 cell using CBR1 Polyclonal Antibody at dilution of 1:1000

CBR1 Polyclonal Antibody

E-AB-14736
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    CBR1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    Carbonyl reductase is one of several monomeric, NADPH-dependent oxidoreductases having wide specificity for carbonyl compounds. This enzyme is widely distributed in human tissues. Another carbonyl reductase gene, CRB3, lies close to this gene on chromosome 21q. NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CBR1 using anti-CBR1 antibody (A02825-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: rat testis tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBR1 antigen affinity purified polyclonal antibody (Catalog # A02825-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBR1 at approximately 36 kDa. The expected band size for CBR1 is at 30 kDa.
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