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ProductsBack/CBS antibody [GT519]

CBS antibody [GT519]

GTX628777
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCBS
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Overview

  • Supplier
    GeneTex
  • Product Name
    CBS antibody [GT519]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT519
  • Concentration
    0.97 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID875
  • Target name
    CBS
  • Target description
    cystathionine beta-synthase
  • Target synonyms
    CBSL, HIP4, cystathionine beta-synthase, Cystathionine beta-synthase-like protein, beta-thionase, methylcysteine synthase, serine sulfhydrase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP0DN79
  • Scientific Description
    The protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zheng J, Sato M, Mishima E, et al. Sorafenib fails to trigger ferroptosis across a wide range of cancer cell lines. Cell Death Dis. 2021,12(7):698. doi: 10.1038/s41419-021-03998-w
    Read this paper
  • Ahmad A, Gerö D, Olah G, et al. Effect of endotoxemia in mice genetically deficient in cystathionine-γ-lyase, cystathionine-β-synthase or 3-mercaptopyruvate sulfurtransferase. Int J Mol Med. 2016,38(6):1683-1692. doi: 10.3892/ijmm.2016.2771
    Read this paper
  • Pincini A, Tornillo G, Orso F, et al. Identification of p130Cas/ErbB2-dependent invasive signatures in transformed mammary epithelial cells. Cell Cycle. 2013,12(15):2409-22. doi: 10.4161/cc.25415
    Read this paper

Datasheet

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Figure 1. Western blot analysis of CBS using anti-CBS antibody (A00130-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: huamn HEK293 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: rat pancreas tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBS antigen affinity purified polyclonal antibody (Catalog # A00130-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CBS at approximately 63 kDa. The expected band size for CBS is at 63 kDa.
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