![CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 30 μg Raji whole cell lysate/extract B. 30 μg K562 whole cell lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] (GTX628777) dilution: 1:1000 CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 30 μg Raji whole cell lysate/extract B. 30 μg K562 whole cell lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] (GTX628777) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX628777/GTX628777_41183_WB_w_23061202_278.webp)
CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 30 μg Raji whole cell lysate/extract B. 30 μg K562 whole cell lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] (GTX628777) dilution: 1:1000
CBS antibody [GT519]
GTX628777
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetCBS
Overview
- SupplierGeneTex
- Product NameCBS antibody [GT519]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT519
- Concentration0.97 mg/ml
- ConjugateUnconjugated
- Gene ID875
- Target nameCBS
- Target descriptioncystathionine beta-synthase
- Target synonymsCBSL, HIP4, cystathionine beta-synthase, Cystathionine beta-synthase-like protein, beta-thionase, methylcysteine synthase, serine sulfhydrase
- HostMouse
- IsotypeIgG1
- Protein IDP0DN79
- Scientific DescriptionThe protein encoded by this gene acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zheng J, Sato M, Mishima E, et al. Sorafenib fails to trigger ferroptosis across a wide range of cancer cell lines. Cell Death Dis. 2021,12(7):698. doi: 10.1038/s41419-021-03998-wRead this paper
- Ahmad A, Gerö D, Olah G, et al. Effect of endotoxemia in mice genetically deficient in cystathionine-γ-lyase, cystathionine-β-synthase or 3-mercaptopyruvate sulfurtransferase. Int J Mol Med. 2016,38(6):1683-1692. doi: 10.3892/ijmm.2016.2771Read this paper
- Pincini A, Tornillo G, Orso F, et al. Identification of p130Cas/ErbB2-dependent invasive signatures in transformed mammary epithelial cells. Cell Cycle. 2013,12(15):2409-22. doi: 10.4161/cc.25415Read this paper
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628777/GTX628777_41183_20180914_WB_B_w_23061202_279.webp)
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:500. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628777/GTX628777_41183_20160922_WB_shRNA_watermark_w_23061202_182.webp)
![CBS antibody [GT519] detects CBS protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. CBS antibody [GT519] (GTX628777) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min CBS antibody [GT519] detects CBS protein at cytoplasm in rat liver by immunohistochemical analysis. Sample: Paraffin-embedded rat liver. CBS antibody [GT519] (GTX628777) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX628777/GTX628777_41183_20160616_IHC-P_R_w_23061202_785.webp)
![CBS antibody [GT519] detects CBS protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CBS antibody [GT519] (GTX628777) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min CBS antibody [GT519] detects CBS protein at cytoplasm in mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CBS antibody [GT519] (GTX628777) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX628777/GTX628777_41183_20160616_IHC-P_M_w_23061202_950.webp)
![CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 50 μg mouse liver lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] (GTX628777) dilution: 1:2000 CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 50 μg mouse liver lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] (GTX628777) dilution: 1:2000](https://www.genetex.com/upload/website/prouct_img/normal/GTX628777/GTX628777_40128_WB_M_liver_w_23061202_497.webp)
![Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Wild-type (WT) and CBS knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628777/GTX628777_41183_20170629_WB_KO_watermark_w_23061202_128.webp)
![CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 50 μg rat liver lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] (GTX628777) dilution: 1:1000 CBS antibody [GT519] detects CBS protein by Western blot analysis. A. 50 μg rat liver lysate/extract 7.5 % SDS-PAGE CBS antibody [GT519] (GTX628777) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX628777/GTX628777_40128_WB_R_liver_w_23061202_557.webp)
![Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Various tissue extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CBS antibody [GT519] (GTX628777) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX628777/GTX628777_41183_20231215_WB_Z_tissue_23121922_720.webp)





