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ProductsBack/CCL3 / MIP-1-Alpha Antibody (aa24-92)

CCL3 / MIP-1-Alpha Antibody (aa24-92)

LS-C314382
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetCCL3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CCL3 / MIP-1-Alpha Antibody (aa24-92)
  • Delivery Days Customer
    23
  • Application Supplier Note
    ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:100 - 1:400) ELISA 1:100-1:5000; ICC 1:50-500; WB 1:50-400;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6348
  • Target name
    CCL3
  • Target description
    C-C motif chemokine ligand 3
  • Target synonyms
    C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; G0S19-1; LD78ALPHA; macrophage inflammatory protein 1-alpha; MIP1A; MIP-1-alpha; PAT 464.1; SCYA3; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); tonsillar lymphocyte LD78 alpha protein
  • Host
    Rabbit
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL3 using anti-CCL3 antibody (PB9571). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9571) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 11 kDa. The expected band size for CCL3 is at 11 kDa.
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