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ProductsBack/MIP1 alpha antibody [199]

MIP1 alpha antibody [199]

GTX18690
GeneTex
ApplicationsELISA
DownloadDatasheet
Product group Antibodies
TargetCCL3
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Overview

  • Supplier
    GeneTex
  • Product Name
    MIP1 alpha antibody [199]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ELISA: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    199
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6348
  • Target name
    CCL3
  • Target description
    C-C motif chemokine ligand 3
  • Target synonyms
    C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; G0S19-1; LD78ALPHA; macrophage inflammatory protein 1-alpha; MIP1A; MIP-1-alpha; PAT 464.1; SCYA3; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); tonsillar lymphocyte LD78 alpha protein
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP10147
  • Protein Name
    C-C motif chemokine 3
  • Scientific Description
    This locus represents a small inducible cytokine. The encoded protein, also known as macrophage inflammatory protein 1 alpha, plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. Polymorphisms at this locus may be associated with both resistance and susceptibility to infection by human immunodeficiency virus type 1.[provided by RefSeq, Sep 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL3 using anti-CCL3 antibody (PB9571). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9571) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 11 kDa. The expected band size for CCL3 is at 11 kDa.
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WB analysis of mouse liver tissue lysate (35ug/lane) using GTX81195 MIP1 alpha antibody, C-term.
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