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ProductsBack/Goat anti-CCL3 and CCL3L1

Goat anti-CCL3 and CCL3L1

EB06650
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetCCL3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-CCL3 and CCL3L1 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID6348
  • Target name
    CCL3
  • Target description
    C-C motif chemokine ligand 3
  • Target synonyms
    C-C motif chemokine 3; G0/G1 switch regulatory protein 19-1; G0S19-1; LD78ALPHA; macrophage inflammatory protein 1-alpha; MIP1A; MIP-1-alpha; PAT 464.1; SCYA3; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); tonsillar lymphocyte LD78 alpha protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002974.1; NP_066286.1. Purification: Antigen affinity purified. Names and symbols: CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3; LD78 alpha beta; Small inducible cytokine A3; small inducible cytokine A3 (homologous to mouse Mip-1a); CCL3L1; LD78; 464.2; SCYA3L; G0S19-2; SCYA3L1; D17S1718; LD78BETA;
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCL3 using anti-CCL3 antibody (PB9571). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: JURKAT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL3 antigen affinity purified polyclonal antibody (Catalog # PB9571) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL3 at approximately 11 kDa. The expected band size for CCL3 is at 11 kDa.
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