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CCL5 Antibody Pair

CSB-EAP05955
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Guinea Pig, Goat, Human, Mouse, Porcine, Rabbit, Sheep
TargetCCL5
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCL5 Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 1.4ug/ml and the detection antibody at a concentration of 1ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID6352
  • Target name
    CCL5
  • Target description
    C-C motif chemokine ligand 5
  • Target synonyms
    D17S136E, RANTES, SCYA5, SIS-delta, SISd, TCP228, eoCP, C-C motif chemokine 5, T-cell specific protein p288, beta-chemokine RANTES, chemokine (C-C motif) ligand 5, eosinophil chemotactic cytokine, regulated upon activation, normally T-expressed, and presumably secreted, small inducible cytokine subfamily A (Cys-Cys), member 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13501
  • Protein Name
    C-C motif chemokine 5
  • Reactivity
    Bovine, Canine, Guinea Pig, Goat, Human, Mouse, Porcine, Rabbit, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of RANTES using anti-RANTES antibody (PB9573). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HUT Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANTES antigen affinity purified polyclonal antibody (Catalog # PB9573) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RANTES at approximately 11 kDa. The expected band size for RANTES is at 11 kDa.
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