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ProductsBack/CCNA1 / Cyclin A1 Antibody (aa64-263)

CCNA1 / Cyclin A1 Antibody (aa64-263)

LS-C407697
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Hamster, Human, Mammals, Mouse, Porcine, Rat, Sheep
TargetCCNA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CCNA1 / Cyclin A1 Antibody (aa64-263)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8900
  • Target name
    CCNA1
  • Target description
    cyclin A1
  • Target synonyms
    CT146, cyclin-A1, testicular tissue protein Li 34
  • Host
    Rabbit
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Hamster, Human, Mammals, Mouse, Porcine, Rat, Sheep
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cyclin A1/CCNA1 using anti-Cyclin A1/CCNA1 antibody (PB9485). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U937 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A1/CCNA1 antigen affinity purified polyclonal antibody (Catalog # PB9485) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A1/CCNA1 at approximately 55 kDa. The expected band size for Cyclin A1/CCNA1 is at 52 kDa.
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