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Western Blot Positive WB detected in: Mouse liver tissue All lanes: CCR3 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution predicted band size: 42, 44 kDa observed band size: 42 kDa
Western Blot Positive WB detected in: Mouse liver tissue All lanes: CCR3 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution predicted band size: 42, 44 kDa observed band size: 42 kDa
Western Blot Positive WB detected in: Mouse liver tissue All lanes: CCR3 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution predicted band size: 42, 44 kDa observed band size: 42 kDa

CCR3 Antibody

CSB-PA004842LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCCR3
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCR3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1232
  • Target name
    CCR3
  • Target description
    C-C motif chemokine receptor 3
  • Target synonyms
    b-chemokine receptor; C C CKR3; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CC-CKR-3; CCR-3; CD193; chemokine (C-C motif) receptor 3; CKR 3; CKR3; CMKBR3; eosinophil CC chemokine receptor 3; eosinophil eotaxin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51677
  • Protein Name
    C-C chemokine receptor type 3
  • Scientific Description
    Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CCR3 using anti-CCR3 antibody (A01748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human CCRF-CEM whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR3 antigen affinity purified polyclonal antibody (Catalog # A01748-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR3 at approximately 55KD. The expected band size for CCR3 is at 41KD.
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