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ProductsBack/CCR9 Polyclonal Antibody, Biotin Conjugated

CCR9 Polyclonal Antibody, Biotin Conjugated

BS-1788R-BIOTIN
Bioss
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
TargetCCR9
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Overview

  • Supplier
    Bioss
  • Product Name
    CCR9 Polyclonal Antibody, Biotin Conjugated
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Biotin
  • Gene ID10803
  • Target name
    CCR9
  • Target description
    C-C motif chemokine receptor 9
  • Target synonyms
    CC-CKR-9, CDw199, GPR-9-6, GPR28, C-C chemokine receptor type 9, G protein-coupled receptor 28, chemokine (C-C motif) receptor 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51686
  • Protein Name
    C-C chemokine receptor type 9
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CCR9 using anti-CCR9 antibody (M02348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR9 antigen affinity purified monoclonal antibody (Catalog # M02348) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR9 at approximately 42 kDa. The expected band size for CCR9 is at 42 kDa.
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