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Immunohistochemistry of paraffin-embedded Human liver cancer tissue using CCR9 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using CCR9 Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using CCR9 Polyclonal Antibody at dilution 1:40

CCR9 Polyclonal Antibody

E-AB-14832
Elabscience
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetCCR9
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Overview

  • Supplier
    Elabscience
  • Product Name
    CCR9 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10803
  • Target name
    CCR9
  • Target description
    C-C motif chemokine receptor 9
  • Target synonyms
    CC-CKR-9, CDw199, GPR-9-6, GPR28, C-C chemokine receptor type 9, G protein-coupled receptor 28, chemokine (C-C motif) receptor 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51686
  • Protein Name
    C-C chemokine receptor type 9
  • Scientific Description
    The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCR9 using anti-CCR9 antibody (M02348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR9 antigen affinity purified monoclonal antibody (Catalog # M02348) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR9 at approximately 42 kDa. The expected band size for CCR9 is at 42 kDa.
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