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Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue All lanes: CCR9 antibody at 1microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 41 KDa Observed band size: 43 KDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue All lanes: CCR9 antibody at 1microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 41 KDa Observed band size: 43 KDa
Western Blot Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, HL-60 whole cell lysate, Hela whole cell lysate, U87 whole cell lysate, Mouse brain tissue All lanes: CCR9 antibody at 1microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 41 KDa Observed band size: 43 KDa

CCR9 Recombinant Monoclonal Antibody

CSB-RA004848A0HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetCCR9
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCR9 Recombinant Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4G2
  • Conjugate
    Unconjugated
  • Gene ID10803
  • Target name
    CCR9
  • Target description
    C-C motif chemokine receptor 9
  • Target synonyms
    C-C chemokine receptor type 9; CC-CKR-9; CDw199; chemokine (C-C motif) receptor 9; G protein-coupled receptor 28; GPR28; GPR-9-6
  • Isotype
    IgG
  • Protein IDP51686
  • Protein Name
    C-C chemokine receptor type 9
  • Scientific Description
    Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCR9 using anti-CCR9 antibody (M02348). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat thymus tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR9 antigen affinity purified monoclonal antibody (Catalog # M02348) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCR9 at approximately 42 kDa. The expected band size for CCR9 is at 42 kDa.
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