![ELISA analysis of antigen using GTX60452 CD105 antibody [3A9].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng ELISA analysis of antigen using GTX60452 CD105 antibody [3A9].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng](https://www.genetex.com/upload/website/prouct_img/normal/GTX60452/GTX60452_20170912_ELISA_w_23061123_556.webp)
ELISA analysis of antigen using GTX60452 CD105 antibody [3A9].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng
CD105 antibody [3A9]
GTX60452
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetENG
Overview
- SupplierGeneTex
- Product NameCD105 antibody [3A9]
- Delivery Days Customer9
- Application Supplier NoteWB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID3A9
- ConjugateUnconjugated
- Gene ID2022
- Target nameENG
- Target descriptionendoglin
- Target synonymsEND, HHT1, ORW1, endoglin, CD105 antigen, soluble endoglin
- HostMouse
- IsotypeIgG1
- Protein IDP17813
- Protein NameEndoglin
- Scientific DescriptionThis gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds to the beta1 and beta3 peptides with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia. This gene may also be involved in preeclampsia and several types of cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2013]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![IHC-P analysis of kidney cancer tissue (left) and stomach cancer tissue (right) using GTX60452 CD105 antibody [3A9]. IHC-P analysis of kidney cancer tissue (left) and stomach cancer tissue (right) using GTX60452 CD105 antibody [3A9].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60452/GTX60452_20170912_IHC-P_w_23061123_986.webp)
![FACS analysis of HepG2 cells using GTX60452 CD105 antibody [3A9]. Green : CD105 Purple : negative control FACS analysis of HepG2 cells using GTX60452 CD105 antibody [3A9]. Green : CD105 Purple : negative control](https://www.genetex.com/upload/website/prouct_img/normal/GTX60452/GTX60452_20170912_FACS_w_23061123_630.webp)
![WB analysis of HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate using GTX60452 CD105 antibody [3A9]. WB analysis of HEK293 (1) and CD105(AA: 331-567)-hIgGFc transfected HEK293 (2) cell lysate using GTX60452 CD105 antibody [3A9].](https://www.genetex.com/upload/website/prouct_img/normal/GTX60452/GTX60452_20170912_WB_w_23061123_144.webp)
![ICC/IF analysis of HepG2 cells using GTX60452 CD105 antibody [3A9]. Green : CD105 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments ICC/IF analysis of HepG2 cells using GTX60452 CD105 antibody [3A9]. Green : CD105 Blue: DRAQ5 fluorescent DNA dye Red: Actin filaments](https://www.genetex.com/upload/website/prouct_img/normal/GTX60452/GTX60452_20170912_ICCIF_w_23061123_269.webp)


![FCM analysis of mouse bone marrow cells using GTX00572-08 CD105 antibody [MJ7/18] (PE).](https://www.genetex.com/upload/website/prouct_img/normal/GTX00572-08/GTX00572-08_20250623_FCM_25062300_103.webp)
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD105 antibody [N3C3] (GTX100508) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100508/GTX100508_39932_20151119_WB_w_23060100_822.webp)
![Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD105 antibody [N1N3] (GTX112684) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112684/GTX112684_40492_20151119_WB_w_23060500_755.webp)
![CD105 antibody [N1N3-2] detects CD105 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. CD105 stained by CD105 antibody [N1N3-2] (GTX112685) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX112685/GTX112685_40499_20191115_IHC-P_w_23060500_674.webp)
![FACS analysis of KG1 cells using GTX75142 CD105 antibody [SN6] (PE).](https://www.genetex.com/upload/website/prouct_img/normal/GTX75142/GTX75142_245_FACS_w_23061322_133.webp)
