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IHC-P analysis of Bouins solution fixed mouse kidney section using GTX53121 CD105 antibody [6J9].
IHC-P analysis of Bouins solution fixed mouse kidney section using GTX53121 CD105 antibody [6J9].
IHC-P analysis of Bouins solution fixed mouse kidney section using GTX53121 CD105 antibody [6J9].

CD105 antibody [6J9]

GTX53121
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetEng
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD105 antibody [6J9]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:400. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6J9
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID13805
  • Target name
    Eng
  • Target description
    endoglin
  • Target synonyms
    AI528660; AI662476; CD105; cell surface MJ7/18 antigen; En; Endo; endoglin; S-endoglin; transmembrane glycoprotein
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDQ63961
  • Protein Name
    Endoglin
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CD105 using anti-CD105 antibody (A02997-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD105 antigen affinity purified polyclonal antibody (Catalog # A02997-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD105 at approximately 95-105KD. The expected band size for CD105 is at 71KD.
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