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IHC-P analysis of rat lung tissue using GTX04685 CD11c antibody. Antigen retrieval : Heat mediated EDTA buffer (pH 8.0) Dilution : 2 microg/mL
IHC-P analysis of rat lung tissue using GTX04685 CD11c antibody. Antigen retrieval : Heat mediated EDTA buffer (pH 8.0) Dilution : 2 microg/mL
IHC-P analysis of rat lung tissue using GTX04685 CD11c antibody. Antigen retrieval : Heat mediated EDTA buffer (pH 8.0) Dilution : 2 microg/mL

CD11c antibody

GTX04685
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetItgax
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD11c antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.25-0.5 microg/mL. IHC-P: 2-5 microg/mL. ELISA: 0.1-0.5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID16411
  • Target name
    Itgax
  • Target description
    integrin alpha X
  • Target synonyms
    Cd11c, Cr4, N418, integrin alpha-X, CD11 antigen-like family member C, CD11C (p150) alpha polypeptide, complement receptor 4, integrin aX, leukocyte adhesion glycoprotein p150,95 alpha chain, leukocyte adhesion receptor p150,95
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD11c/Itgax using anti-CD11c/Itgax antibody (A00357-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11c/Itgax antigen affinity purified polyclonal antibody (Catalog # A00357-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD11c/Itgax at approximately 127 kDa. The expected band size for CD11c/Itgax is at 127 kDa.
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