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ProductsBack/CD13 (ANPEP) Mouse Monoclonal Antibody [Clone ID: CVS19]
Staining of equine peripheral blood granulocytes with mouse anti horse CD13 clone CVS19. followed by secondary FITC conjugated antibody.
Staining of equine peripheral blood granulocytes with mouse anti horse CD13 clone CVS19. followed by secondary FITC conjugated antibody.
Staining of equine peripheral blood granulocytes with mouse anti horse CD13 clone CVS19. followed by secondary FITC conjugated antibody.

CD13 (ANPEP) Mouse Monoclonal Antibody [Clone ID: CVS19]

AM01047PU-S
OriGene
ApplicationsFlow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetANPEP
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    CD13 (ANPEP) Mouse Monoclonal Antibody [Clone ID: CVS19]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    CVS19
  • Gene ID290
  • Target name
    ANPEP
  • Target description
    alanyl aminopeptidase, membrane
  • Target synonyms
    AP-M, AP-N, APN, CD13, GP150, LAP1, P150, PEPN, hAPN, aminopeptidase N, alanyl (membrane) aminopeptidase, aminopeptidase M, membrane alanyl aminopeptidase, microsomal aminopeptidase, myeloid plasma membrane glycoprotein CD13
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15144
  • Protein Name
    Aminopeptidase N
  • Scientific Description
    CD13 (ANPEP) mouse monoclonal antibody, clone CVS19, Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of ANPEP using anti-ANPEP antibody (A02591-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANPEP antigen affinity purified polyclonal antibody (Catalog # A02591-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANPEP at approximately 150KD. The expected band size for ANPEP is at 150KD.
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