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ProductsBack/MOUSE ANTI CAT CD134

MOUSE ANTI CAT CD134

ORB433894
Biorbyt
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
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Overview

  • Supplier
    Biorbyt
  • Product Name
    CD134 antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    FC, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7D6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    Mouse monoclonal antibody to CD134
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD134 using anti-CD134 antibody (PB9898). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates, Lane 2: 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134 antigen affinity purified polyclonal antibody (Catalog # PB9898) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134 at approximately 50KD. The expected band size for CD134 is at 50KD.
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