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Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CD134 Polyclonal Antibody, Unconjugated (bs-2685R) at 1:200 overnight at 4°C, DAB staining.

CD134 Polyclonal Antibody

BS-2685R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rat
TargetTNFRSF4
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Overview

  • Supplier
    Bioss
  • Product Name
    CD134 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID7293
  • Target name
    TNFRSF4
  • Target description
    TNF receptor superfamily member 4
  • Target synonyms
    ACT35, CD134, IMD16, OX40, TXGP1L, tumor necrosis factor receptor superfamily member 4, ACT35 antigen, ATC35 antigen, CD134 antigen, OX40 antigen, OX40 cell surface antigen, OX40 homologue, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, lymphoid activation antigene ACT35, tax-transcriptionally activated glycoprotein 1 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43489
  • Protein Name
    Tumor necrosis factor receptor superfamily member 4
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of CD134 using anti-CD134 antibody (PB9898). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates, Lane 2: 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134 antigen affinity purified polyclonal antibody (Catalog # PB9898) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134 at approximately 50KD. The expected band size for CD134 is at 50KD.
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