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Confocal microscopy of PFA-fixed mouse splenocytes stained with CD137 Rabbit Recombinant Antibody AE00313 at 10ug/ml for 1h at RT. Detection by confocal microscopy using CF488 (green) for the antibody and DAPI (blue) for nuclear staining. Bottom right shows staining with an isotype control antibody.
Confocal microscopy of PFA-fixed mouse splenocytes stained with CD137 Rabbit Recombinant Antibody AE00313 at 10ug/ml for 1h at RT. Detection by confocal microscopy using CF488 (green) for the antibody and DAPI (blue) for nuclear staining. Bottom right shows staining with an isotype control antibody.
Confocal microscopy of PFA-fixed mouse splenocytes stained with CD137 Rabbit Recombinant Antibody AE00313 at 10ug/ml for 1h at RT. Detection by confocal microscopy using CF488 (green) for the antibody and DAPI (blue) for nuclear staining. Bottom right shows staining with an isotype control antibody.

CD137 Recombinant Antibody AE00313

AE00313
Aeonian Biotech
TargetTnfrsf9
Product group Antibodies
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Overview

  • Supplier
    Aeonian Biotech
  • Product Name
    CD137 Recombinant Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    FC, ICC, CD8+ T cell activation
  • Category Supplier
    Antibody
  • Certification
    Research Use Only
  • Clonality
    Recombinant
  • Clone ID
    LOB12.3R
  • Conjugate
    Unconjugated
  • Gene ID21942
  • Target name
    Tnfrsf9
  • Target description
    tumor necrosis factor receptor superfamily, member 9
  • Target synonyms
    4-1BB; 4-1BB ligand receptor; A930040I11Rik; AA408498; AI325004; Cd137; CD137 antigen; CDw137; ILA; Ly6; Ly63; secreted CD137 antigen; T-cell antigen 4-1BB; tumor necrosis factor receptor superfamily member 9
  • Host
    Rabbit
  • Isotype
    IgG2a kappa
  • Protein IDP20334
  • Protein Name
    Tumor necrosis factor receptor superfamily member 9
  • Scientific Description
    CD137 Recombinant Antibody AE00313
  • Shelf life instruction
    Integrity warranted for 24 months after purchase when handled and stored according to instructions, see below.
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2-8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD137 using anti-CD137 antibody (A00867-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant mouse CD137 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD137 antigen affinity purified polyclonal antibody (Catalog # A00867-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD137 at approximately 65KD. The expected band size for CD137 is at 44KD.
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