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FACS analysis of 10? RAW264.7 cells using GTX54397 CD14 antibody [Sa14-2]. Black : No ab staining Red : Isotype control Blue : Primary antibody Dilution : 1microg/ml
FACS analysis of 10? RAW264.7 cells using GTX54397 CD14 antibody [Sa14-2]. Black : No ab staining Red : Isotype control Blue : Primary antibody Dilution : 1microg/ml
FACS analysis of 10? RAW264.7 cells using GTX54397 CD14 antibody [Sa14-2]. Black : No ab staining Red : Isotype control Blue : Primary antibody Dilution : 1microg/ml

CD14 antibody [Sa14-2]

GTX54397
GeneTex
ApplicationsFunctional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetCd14
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD14 antibody [Sa14-2]
  • Delivery Days Customer
    9
  • Applications
    Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Sa14-2
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID12475
  • Target name
    Cd14
  • Target description
    CD14 antigen
  • Target synonyms
    monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein
  • Host
    Rat
  • Isotype
    IgG2a
  • Protein IDP10810
  • Protein Name
    Monocyte differentiation antigen CD14
  • Scientific Description
    This gene encodes a protein that plays an important role in the innate immune response and is expressed in monocyte/macrophage cells. This gene product acts as a co-receptor that binds several microbial and fungal molecules, including lipopolysaccharides (LPS). This proteins LPS-binding activity is enhanced by the LPS binding protein (LBP) to allow binding to the TLR4-MD-2 co-receptor complex. The product of this gene is found in two forms, either as a soluble protein or attached to the cell surface by a glycosylphosphatidylinositol anchor. [provided by RefSeq, Jul 2014]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cd14 using anti-Cd14 antibody (A00137-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd14 antigen affinity purified polyclonal antibody (Catalog # A00137-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd14 at approximately 50 kDa. The expected band size for Cd14 is at 39 kDa.
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