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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD14 Polyclonal Antibody, Unconjugated (bs-1192R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

CD14 Polyclonal Antibody

BS-1192R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCd14
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Overview

  • Supplier
    Bioss
  • Product Name
    CD14 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID12475
  • Target name
    Cd14
  • Target description
    CD14 antigen
  • Target synonyms
    monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10810
  • Protein Name
    Monocyte differentiation antigen CD14
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ma WJ, Wu ZR, Yang Q, et al. Biliary antibiotics irrigation for E. coli-induced chronic proliferative cholangitis and hepatolithiasis: A pathophysiological study in rabbits. Clin Res Hepatol Gastroenterol. 2020,44(3):356-367. doi: 10.1016/j.clinre.2019.07.008
    Read this paper
  • Liu GZ, Zhang S, Li YY, et al. Aldosterone stimulation mediates cardiac metabolism remodeling via Sirt1/AMPK signaling in canine model. Naunyn Schmiedebergs Arch Pharmacol. 2019,392(7):851-863. doi: 10.1007/s00210-019-01641-2
    Read this paper
  • Sheu JJ, Lee MS, Wallace CG, et al. Therapeutic effects of adipose derived fresh stromal vascular fraction-containing stem cells versus cultured adipose derived mesenchymal stem cells on rescuing heart function in rat after acute myocardial infarction. Am J Transl Res. 2019,11(1):67-86.
    Read this paper
  • Luo Y, Lu J, Ruan W, et al. MCC950 attenuated early brain injury by suppressing NLRP3 inflammasome after experimental SAH in rats. Brain Res Bull. 2019,146:320-326. doi: 10.1016/j.brainresbull.2019.01.027
    Read this paper
  • Liu Y, Yang D, Wei S, et al. Isolation and characterization of ovine monocyte-derived macrophages from peripheral blood. Vet Immunol Immunopathol. 2018,205:83-92. doi: 10.1016/j.vetimm.2018.11.004
    Read this paper
  • Sahiner IF, Soylu H, Ates E, et al. Impact of intravesical hyaluronic acid treatment on bladder inflammation in interstitial cystitis rat model. Int Braz J Urol. 2018,44(5):1014-1022. doi: 10.1590/S1677-5538.IBJU.2017.0713
    Read this paper
  • Sung PH, Lee FY, Lin LC, et al. Melatonin attenuated brain death tissue extract-induced cardiac damage by suppressing DAMP signaling. Oncotarget. 2018,9(3):3531-3548. doi: 10.18632/oncotarget.23180
    Read this paper
  • Kulhankova K, Kinney KJ, Stach JM, et al. The Superantigen Toxic Shock Syndrome Toxin 1 Alters Human Aortic Endothelial Cell Function. Infect Immun. 2018,86(3). doi: 10.1128/IAI.00848-17
    Read this paper
  • Lee FY, Chen KH, Wallace CG, et al. Xenogeneic human umbilical cord-derived mesenchymal stem cells reduce mortality in rats with acute respiratory distress syndrome complicated by sepsis. Oncotarget. 2017,8(28):45626-45642. doi: 10.18632/oncotarget.17320
    Read this paper
  • Sun CK, Chen CH, Chang CL, et al. Melatonin treatment enhances therapeutic effects of exosomes against acute liver ischemia-reperfusion injury. Am J Transl Res. 2017,9(4):1543-1560.
    Read this paper

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Figure 1. Western blot analysis of Cd14 using anti-Cd14 antibody (A00137-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd14 antigen affinity purified polyclonal antibody (Catalog # A00137-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd14 at approximately 50 kDa. The expected band size for Cd14 is at 39 kDa.
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