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ProductsBack/FITC Anti-Mouse CD14 Antibody [Sa14-2]

FITC Anti-Mouse CD14 Antibody [Sa14-2]

AGEL1863
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityMouse
TargetCd14
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Overview

  • Supplier
    Assay Genie
  • Product Name
    FITC Anti-Mouse CD14 Antibody [Sa14-2]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    Sa14-2
  • Conjugate
    FITC
  • Gene ID12475
  • Target name
    Cd14
  • Target description
    CD14 antigen
  • Target synonyms
    monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein
  • Host
    Rat
  • Protein IDP10810
  • Protein Name
    Monocyte differentiation antigen CD14
  • Scientific Description
    FITC Anti-Mouse CD14 Antibody [Sa14-2] is a high-performance monoclonal that specifically targets Mouse CD14, delivering exceptional specificity and reliability for your research applications. Rigorously validated for FCM, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100 Tests format to meet your experimental needs. For optimal performance, store at 4°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Cd14 using anti-Cd14 antibody (A00137-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cd14 antigen affinity purified polyclonal antibody (Catalog # A00137-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cd14 at approximately 50 kDa. The expected band size for Cd14 is at 39 kDa.
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