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Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (bs-4690RR) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (bs-4690RR) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (bs-4690RR) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Tissue factor Polyclonal Antibody

BS-4690R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetF3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CD142 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2152
  • Target name
    F3
  • Target description
    coagulation factor III, tissue factor
  • Target synonyms
    CD142; coagulation factor III (thromboplastin, tissue factor); TF; TFA; tissue factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13726
  • Protein Name
    Tissue factor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution. Fletcher RB et al., 2017 Jun 1, Cell Stem Cell
    Read more
  • Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy. Gleeson BM et al., 2015 Sep, Stem Cells
    Read more

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Figure 1. Western blot analysis of Tissue Factor using anti-Tissue Factor antibody (PB9701). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tissue Factor antigen affinity purified polyclonal antibody (Catalog # PB9701) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tissue Factor at approximately 50 kDa. The expected band size for Tissue Factor is at 33 kDa.
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