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Western Blot analysis of COS7 cells using TF Polyclonal Antibody
Western Blot analysis of COS7 cells using TF Polyclonal Antibody
Western Blot analysis of COS7 cells using TF Polyclonal Antibody

F3 Antibody

CSB-PA004263
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey
TargetF3
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Overview

  • Supplier
    Cusabio
  • Product Name
    F3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2152
  • Target name
    F3
  • Target description
    coagulation factor III, tissue factor
  • Target synonyms
    CD142, TF, TFA, tissue factor, coagulation factor III (thromboplastin, tissue factor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13726
  • Protein Name
    Tissue factor
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Tissue Factor using anti-Tissue Factor antibody (PB9701). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tissue Factor antigen affinity purified polyclonal antibody (Catalog # PB9701) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tissue Factor at approximately 50 kDa. The expected band size for Tissue Factor is at 33 kDa.
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