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ProductsBack/CD147 antibody [13F31]

CD147 antibody [13F31]

GTX53320
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityMouse
TargetBsg
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD147 antibody [13F31]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    13F31
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID12215
  • Target name
    Bsg
  • Target description
    basigin
  • Target synonyms
    CD147, EMMPRIN, HT-7, basigin, 5A11/Basigin, HT7 antigen, basic immunoglobulin superfamily, gp 42, membrane glycoprotein gp42, neurothelin
  • Host
    Rat
  • Isotype
    IgG2
  • Protein IDP18572
  • Protein Name
    Basigin
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD147/Bsg using anti-CD147/Bsg antibody (A00248-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD147/Bsg antigen affinity purified polyclonal antibody (Catalog # A00248-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD147/Bsg at approximately 45 kDa. The expected band size for CD147/Bsg is at 45 kDa.
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