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Lovo cell lysates probed with CD15 Polyclonal Antibody, Unconjugated (bs-20726R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lovo cell lysates probed with CD15 Polyclonal Antibody, Unconjugated (bs-20726R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lovo cell lysates probed with CD15 Polyclonal Antibody, Unconjugated (bs-20726R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

FUT4 Polyclonal Antibody

BS-20726R
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot, ELISA
DownloadDatasheet
Product group Antibodies
TargetFUT4
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CD15 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2526
  • Target name
    FUT4
  • Target description
    fucosyltransferase 4
  • Target synonyms
    alpha (1,3) fucosyltransferase, myeloid-specific; alpha-(1,3)-fucosyltransferase 4; CD15; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; ELFT; FCT3A; fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA-1; stage-specific embryonic antigen 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP22083
  • Protein Name
    Alpha-(1,3)-fucosyltransferase 4
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FUT4 using anti-FUT4 antibody (A04535-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FUT4 antigen affinity purified polyclonal antibody (Catalog # A04535-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FUT4 at approximately 70KD. The expected band size for FUT4 is at 70KD.
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