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FUT4 / CD15 Antibody (clone HI98)

LS-C769994
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoHistoChemistry
Product group Antibodies
TargetFUT4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FUT4 / CD15 Antibody (clone HI98)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.5 microg per 10E6 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.. Flo, IHC Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.5 µg per 10E6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
  • Applications
    Flow Cytometry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HI98
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2526
  • Target name
    FUT4
  • Target description
    fucosyltransferase 4
  • Target synonyms
    alpha (1,3) fucosyltransferase, myeloid-specific; alpha-(1,3)-fucosyltransferase 4; CD15; ELAM ligand fucosyltransferase; ELAM-1 ligand fucosyltransferase; ELFT; FCT3A; fucosyltransferase IV; FUC-TIV; fucT-IV; FUTIV; galactoside 3-L-fucosyltransferase; Lewis X; LeX; SSEA-1; stage-specific embryonic antigen 1
  • Host
    Mouse
  • Isotype
    IgM
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FUT4 using anti-FUT4 antibody (A04535-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FUT4 antigen affinity purified polyclonal antibody (Catalog # A04535-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FUT4 at approximately 70KD. The expected band size for FUT4 is at 70KD.
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