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ProductsBack/Purified Anti-Human CD15 Antibody [HI98]

Purified Anti-Human CD15 Antibody [HI98]

AGEL0296
Assay Genie
ApplicationsFlow Cytometry, ImmunoHistoChemistry
Product group Antibodies
TargetFUT4
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Purified Anti-Human CD15 Antibody [HI98]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoHistoChemistry
  • Applications Supplier
    FCM,IHC
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2526
  • Target name
    FUT4
  • Target description
    fucosyltransferase 4
  • Target synonyms
    CD15, ELFT, FCT3A, FUC-TIV, FUTIV, LeX, SSEA-1, alpha-(1,3)-fucosyltransferase 4, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, ELAM ligand fucosyltransferase, ELAM-1 ligand fucosyltransferase, Lewis X, alpha (1,3) fucosyltransferase, myeloid-specific, fucT-IV, fucosyltransferase IV, galactoside 3-L-fucosyltransferase, stage-specific embryonic antigen 1
  • Host
    Mouse
  • Protein IDP22083
  • Protein Name
    Alpha-(1,3)-fucosyltransferase 4
  • Shelf life instruction
    12 months
  • Source
    Mouse
  • Reactivity Supplier
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FUT4 using anti-FUT4 antibody (A04535-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FUT4 antigen affinity purified polyclonal antibody (Catalog # A04535-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FUT4 at approximately 70KD. The expected band size for FUT4 is at 70KD.
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