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Mouse spleen lysates probed with CD274 Polyclonal Antibody, unconjugated (bs-1103R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse spleen lysates probed with CD274 Polyclonal Antibody, unconjugated (bs-1103R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse spleen lysates probed with CD274 Polyclonal Antibody, unconjugated (bs-1103R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

PD-L1 Polyclonal Antibody

BS-1103R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCd274
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CD274 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID60533
  • Target name
    Cd274
  • Target description
    CD274 antigen
  • Target synonyms
    A530045L16Rik; B7 homolog 1; B7h1; PD-; PDCD1 ligand 1; Pdcd1l; Pdcd1l1; Pdcd1lg1; Pdl1; programmed cell death 1 ligand 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9EP73
  • Protein Name
    Programmed cell death 1 ligand 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PD-L1/Cd274 using anti-PD-L1/Cd274 antibody (A00109-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PD-L1/Cd274 antigen affinity purified polyclonal antibody (Catalog # A00109-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PD-L1/Cd274 at approximately 40KD. The expected band size for PD-L1/Cd274 is at 40KD.
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