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FACS analysis of human peripheral blood lymphocytes using GTX01460-07 CD3 antibody [SK7] (APC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 μg (5 μl)
FACS analysis of human peripheral blood lymphocytes using GTX01460-07 CD3 antibody [SK7] (APC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 μg (5 μl)
FACS analysis of human peripheral blood lymphocytes using GTX01460-07 CD3 antibody [SK7] (APC). Solid lone : primary antibody Dashed line : isotype control antibody amount : 0.25 μg (5 μl)

CD3 antibody [SK7] (APC)

GTX01460-07
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman, Primate
TargetCD3D
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD3 antibody [SK7] (APC)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 0.25 microg (5 microl) for 105-108 cells in 100 microl sample per test. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SK7
  • Concentration
    0.05 mg/ml
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID915
  • Target name
    CD3D
  • Target description
    CD3 delta subunit of T-cell receptor complex
  • Target synonyms
    CD3-DELTA, CD3DELTA, IMD19, T3D, T-cell surface glycoprotein CD3 delta chain, CD3 antigen, delta subunit, CD3 delta, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), OKT3, delta chain, T-cell receptor T3 delta chain
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04234
  • Protein Name
    T-cell surface glycoprotein CD3 delta chain
  • Reactivity
    Human, Primate
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Wrann CD, Laue T, Hübner L, et al. Short-term and long-term leptin exposure differentially affect human natural killer cell immune functions. Am J Physiol Endocrinol Metab. 2012,302(1):E108-16. doi: 10.1152/ajpendo.00057.2011
    Read this paper
  • Soto PC, Stein LL, Hurtado-Ziola N, et al. Relative over-reactivity of human versus chimpanzee lymphocytes: implications for the human diseases associated with immune activation. J Immunol. 2010,184(8):4185-95. doi: 10.4049/jimmunol.0903420
    Read this paper
  • Goval JJ, Greimers R, Boniver J, et al. Germinal center dendritic cells express more ICAM-1 than extrafollicular dendritic cells and ICAM-1/LFA-1 interactions are involved in the capacity of dendritic cells to induce PBMCs proliferation. J Histochem Cytochem. 2006,54(1):75-84.
    Read this paper
  • Haringman JJ, Vinkenoog M, Gerlag DM, et al. Reliability of computerized image analysis for the evaluation of serial synovial biopsies in randomized controlled trials in rheumatoid arthritis. Arthritis Res Ther. 2005,7(4):R862-7.
    Read this paper
  • Kaneoka H, Perez-Rojas G, Sasasuki T, et al. Human T lymphocyte proliferation induced by a pan-T monoclonal antibody (anti-Leu 4): heterogeneity of response is a function of monocytes. J Immunol. 1983,131(1):158-64.
    Read this paper

Datasheet

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Figure 1. Western blot analysis of CD3D using anti-CD3D antibody (A04405-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human CCRF-CEM whole cell lysates, Lane 3: human MOLT-4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3D antigen affinity purified polyclonal antibody (Catalog # A04405-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3D at approximately 19 kDa. The expected band size for CD3D is at 19 kDa.
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