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ProductsBack/CD3 epsilon antibody [145-2C11]

CD3 epsilon antibody [145-2C11]

GTX16181
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking, Other Application
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Product group Antibodies
TargetCd3e
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD3 epsilon antibody [145-2C11]
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, Neutralisation/Blocking, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    145-2c11
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID12501
  • Target name
    Cd3e
  • Target description
    CD3 antigen, epsilon polypeptide
  • Target synonyms
    AI504783; CD3; CD3epsilon; T3e; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain
  • Host
    Hamster
  • Isotype
    IgG1
  • Protein IDP22646
  • Protein Name
    T-cell surface glycoprotein CD3 epsilon chain
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Simula L et al., 2018 Dec 11, Cell Rep
    Read more
  • In vivo depletion of leukocytes and platelets following injection of T cell-specific antibodies into mice. Loubaki L et al., 2013 Jul 31, J Immunol Methods
    Read more
  • Signaling through C5a receptor and C3a receptor diminishes function of murine natural regulatory T cells. Kwan WH et al., 2013 Feb 11, J Exp Med
    Read more
  • Anti-CD3epsilon induces splenic B220lo B-cell expansion following anti-CD20 treatment in a mouse model of allosensitization. Todo T et al., 2012 Aug, Int Immunol
    Read more
  • NLRC5 deficiency selectively impairs MHC class I- dependent lymphocyte killing by cytotoxic T cells. Staehli F et al., 2012 Apr 15, J Immunol
    Read more
  • WASp-deficient B cells play a critical, cell-intrinsic role in triggering autoimmunity. Becker-Herman S et al., 2011 Sep 26, J Exp Med
    Read more
  • Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help. Nagashima T et al., 2011 Jan, Am J Pathol
    Read more
  • Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas. Salmond RJ et al., 2011 Jan 6, Blood
    Read more
  • Statins induce regulatory T cell recruitment via a CCL1 dependent pathway. Mira E et al., 2008 Sep 1, J Immunol
    Read more
  • Identification of a monoclonal antibody specific for a murine T3 polypeptide. Leo O et al., 1987 Mar, Proc Natl Acad Sci U S A
    Read more

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Figure 1. Western blot analysis of CD3 epsilon/Cd3e using anti-CD3 epsilon/Cd3e antibody (A02765-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3 epsilon/Cd3e antigen affinity purified polyclonal antibody (Catalog # A02765-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD3 epsilon/Cd3e at approximately 23 kDa. The expected band size for CD3 epsilon/Cd3e is at 21 kDa.
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