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Western blot All lanes: CNTN4 antibody IgG at 2.22ug/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 21, 12, 17 kDa Observed band size: 34 kDa
Western blot All lanes: CNTN4 antibody IgG at 2.22ug/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 21, 12, 17 kDa Observed band size: 34 kDa
Western blot All lanes: CNTN4 antibody IgG at 2.22ug/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 34, 21, 12, 17 kDa Observed band size: 34 kDa

CD300A Antibody

CSB-PA887023ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCD300A
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD300A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID11314
  • Target name
    CD300A
  • Target description
    CD300a molecule
  • Target synonyms
    CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9, CMRF35H, CMRF35H9, IGSF12, IRC1, IRC1/IRC2, IRC2, IRp60, CMRF35-like molecule 8, CD300 antigen-like family member A, CD300a antigen, CMRF35H leukocyte immunoglobulin-like receptor, NK inhibitory receptor, immunoglobulin superfamily member 12, inhibitory receptor protein 60, leukocyte membrane antigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UGN4
  • Protein Name
    CMRF35-like molecule 8
  • Scientific Description
    Inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD300A using anti-CD300A antibody (A05953-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Raw264.7 whole cell lysates, Lane 6: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD300A antigen affinity purified polyclonal antibody (Catalog # A05953-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD300A at approximately 68 kDa. The expected band size for CD300A is at 33 kDa.
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