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ProductsBack/CD300a antibody [MEM-260] (PE)
Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) PE.
Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) PE.
Surface staining of human peripheral blood cells with anti-CD300a (MEM-260) PE.

CD300a antibody [MEM-260] (PE)

GTX79961
GeneTex
ApplicationsFlow Cytometry
Product group Antibodies
ReactivityHuman
TargetCD300A
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD300a antibody [MEM-260] (PE)
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 20 microl reagent / 100 microl of whole blood or 106 cells in a suspension. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MEM-260
  • Conjugate
    RPE
  • Gene ID11314
  • Target name
    CD300A
  • Target description
    CD300a molecule
  • Target synonyms
    CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9, CMRF35H, CMRF35H9, IGSF12, IRC1, IRC1/IRC2, IRC2, IRp60, CMRF35-like molecule 8, CD300 antigen-like family member A, CD300a antigen, CMRF35H leukocyte immunoglobulin-like receptor, NK inhibitory receptor, immunoglobulin superfamily member 12, inhibitory receptor protein 60, leukocyte membrane antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9UGN4
  • Protein Name
    CMRF35-like molecule 8
  • Scientific Description
    This gene encodes a member of the CD300 glycoprotein family of cell surface proteins found on leukocytes involved in immune response signaling pathways. This gene is located on chromosome 17 in a cluster with all but one of the other family members. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD300A using anti-CD300A antibody (A05953-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse Raw264.7 whole cell lysates, Lane 6: mouse J774A.1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD300A antigen affinity purified polyclonal antibody (Catalog # A05953-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD300A at approximately 68 kDa. The expected band size for CD300A is at 33 kDa.
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