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ProductsBack/CD31 antibody [MEC7.46]
The data was published in the journal Sci Rep in 2018.PMID: 29463847
The data was published in the journal Sci Rep in 2018.PMID: 29463847
The data was published in the journal Sci Rep in 2018.PMID: 29463847

CD31 antibody [MEC7.46]

GTX54379
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetPecam1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD31 antibody [MEC7.46]
  • Delivery Days Customer
    9
  • Application Supplier Note
    *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    MEC7.46
  • Concentration
    100 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID18613
  • Target name
    Pecam1
  • Target description
    platelet/endothelial cell adhesion molecule 1
  • Target synonyms
    C85791; Cd31; Pecam; PECAM-1; platelet endothelial cell adhesion molecule
  • Host
    Rat
  • Isotype
    IgG1
  • Protein IDQ08481
  • Protein Name
    Platelet endothelial cell adhesion molecule
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

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  • Adaptive multi-degree-of-freedom in situ bioprinting robot for hair-follicle-inclusive skin repair: A preliminary study conducted in mice.
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  • Small-Vessel Vasculopathy Due to Aberrant Autophagy in LAMP-2 Deficiency. Nguyen HT et al., 2018 Feb 20, Sci Rep
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  • MicroRNA regulation of endothelial TREX1 reprograms the tumour microenvironment. Wilson R et al., 2016 Nov 25, Nat Commun
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  • Local injection of deferoxamine improves neovascularization in ischemic diabetic random flap by increasing HIF-1alpha and VEGF expression. Wang C et al., 2014, PLoS One
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  • Adipose stromal cells amplify angiogenic signaling via the VEGF/mTOR/Akt pathway in a murine hindlimb ischemia model: a 3D multimodality imaging study. Fan W et al., 2012, PLoS One
    Read more

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Figure 1. Western blot analysis of CD31 using anti-CD31 antibody (A01513-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD31 antigen affinity purified polyclonal antibody (Catalog # A01513-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD31 at approximately 120-130 kDa. The expected band size for CD31 is at 83 kDa.
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